yy1 d5d9z rabbit monoclonal antibody  (Cell Signaling Technology Inc)

Journal: Redox Biology

Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

doi: 10.1016/j.redox.2026.104085

Figure Lengend Snippet: YY1 positively regulated the expression of ANXA3 at the transcriptional level. (A) The binding ability of YY1 to the ANXA3 promoter in AC16 cells was detected by ChIP assay, n = 6. (B) The ability of YY1 to active the ANXA3 promoter in HEK293 cells was detected by Dual-luciferase reporter assay, n = 6. (C-D) Relative YY1 and ANXA3 mRNA expression levels in AC16 cells analyzed by RT-qPCR, n = 4. (E-H) Relative YY1 and ANXA3 protein expression levels in AC16 cells analyzed by Western blot, n = 5. The data were presented as the mean ± SD. ∗∗P < 0.01 versus the IgG group or the OE-NC group; ∗∗∗P < 0.001 versus the si-NC group (YY1) or the OE-NC group (YY1); # P < 0.05 versus the OE-NC group (ANXA3); ## P < 0.01 versus the OE-NC group (ANXA3) or the si-NC group (ANXA3).

Article Snippet: After restoring the frozen sections to room temperature, blocked them with 5%bovine serum albumin (w/v)for 30 min, then incubated them with anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:200 [v/v]), anti-YY1 antibody (Proteintech, 22156-1-AP; 1:200 [v/v]), anti-LAMP1 antibody (Proteintech, 21997-1-AP; 1:200 [v/v]) and anti-3-NT antibody (Millipore, Massachusetts, 06284; 1:400 [v/v]) for 16 h respectively.

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Western Blot

Journal: Redox Biology

Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

doi: 10.1016/j.redox.2026.104085

Figure Lengend Snippet: T2DM attenuated the function of YY1 by promoting nitrosative stress. (A-B) Relative YY1 protein expression levels in the cardiac tissue of mice analyzed by Western blot, n = 4. (C-D) Relative YY1 protein expression levels in AC16 cells analyzed by Western blot, n = 6. (E-F) Representative images of IF and the statistical chart were used to observe the nuclear translocation situation of YY1 in the cardiac tissue of mice. Bars: 25 μm, n = 4. (G-H) Relative YY1 protein expression levels in the nucleus of AC16 cells analyzed by Western blot, n = 4-5. (I) Representative images of IF were used to observe the expression level of 3-NT in the cardiac tissue of mice. Bars: 25 μm. (J-K) Relative YY1 protein expression levels in the nucleus of AC16 cells analyzed by Western blot, n = 3. The data were presented as the mean ± SD. ∗ P < 0.05 versus the Vehicle group; ∗∗∗ P < 0.001 versus the db/m group; # P < 0.05 versus the HGPA group; ### P < 0.01 versus the HGPA group.

Article Snippet: After restoring the frozen sections to room temperature, blocked them with 5%bovine serum albumin (w/v)for 30 min, then incubated them with anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:200 [v/v]), anti-YY1 antibody (Proteintech, 22156-1-AP; 1:200 [v/v]), anti-LAMP1 antibody (Proteintech, 21997-1-AP; 1:200 [v/v]) and anti-3-NT antibody (Millipore, Massachusetts, 06284; 1:400 [v/v]) for 16 h respectively.

Techniques: Expressing, Western Blot, Translocation Assay

Journal: Redox Biology

Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

doi: 10.1016/j.redox.2026.104085

Figure Lengend Snippet: Tyr185 site of YY1 nitrated was involved in inhibiting the transcription of ANXA3. (A-B) Relative nitration levels of YY1 in HEK293 cells analyzed by IP, n = 7. (C-D) Relative YY1 expression levels in the nucleus of AC16 cells analyzed by Western blot, n = 5. (E-F) Relative YY1 and ANXA3 mRNA expression levels in AC16 cells analyzed by RT-qPCR, n = 6. The data were presented as the mean ± SD. ∗P < 0.05 versus the WT group; ∗∗P < 0.01 versus the WT group; ∗∗∗P < 0.001 versus the WT group; ## P < 0.01 versus the WT + HGPA group.

Article Snippet: After restoring the frozen sections to room temperature, blocked them with 5%bovine serum albumin (w/v)for 30 min, then incubated them with anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:200 [v/v]), anti-YY1 antibody (Proteintech, 22156-1-AP; 1:200 [v/v]), anti-LAMP1 antibody (Proteintech, 21997-1-AP; 1:200 [v/v]) and anti-3-NT antibody (Millipore, Massachusetts, 06284; 1:400 [v/v]) for 16 h respectively.

Techniques: Nitration, Expressing, Western Blot, Quantitative RT-PCR